Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSI---FLALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSPFLNAFESAIVA-PLVEEPLKLLPLVFVLALIPVRKLKFLFLLGIASGLGFQMIEDIGYIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIV------------ 5IMU Chain:A ((4-220)) AGAVSARAAEQQRLQRIVDAVARQEPRISWAAGLRDDGTTTLLVTDLAGGWIPPHVRLPANVTLLEPTARRRDAD---VIDLLGAVVAVAAHES--NTYVAEP----GPDAPALTGDRSARSAIPKVDE----FGPTLVEAVRRRDSLP-----RIAQAIALPAVRKTGVLENE-AELLHGCITAVKESVLKAYPSH----ELTAVGDWMLLAAIEALIDEQDYLANYHLAWYAVTTRRG

General information: TITO was launched using: RESULT: Template: 5IMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 781 4550 5.83 23.21 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 5.83 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_5IMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IMU-query.scw PDB file : Tito_Scwrl_5IMU.pdb: