Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDDIGQFAIWQDRMFGQTAQHGLTNSLKDFWIFLLNIGPQLAFFCQMLRCSRSVEQGTGNHRREFNMIQQIFSHFGMTHLGQIKLVYQESIDLELLVNALNHHLLIDRLVLTPNQITIEIDRQIVHGLDLLKGRKDKEIIDIKSMFRQLELASTQQICPINQRVHHGILAFGEISDLVPAKNLPNRQD 3BJ5 Chain:A ((15-144)) LVIEFTE----------QTAPKIFGGEIKTHILLFLP-----------------------KSVSDYDGKLSNFKTAAESFKGKILFAFID-------SDHTDNQRILEFF---------GLKKEECPAVRLITLEEEMTKYKPES-----EELTAERITEFCHRFLEG-----KIKPHLMSQELPEDWD

General information: TITO was launched using: RESULT: Template: 3BJ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 582 -77460 -133.09 -595.85 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -133.09 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_3BJ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BJ5-query.scw PDB file : Tito_Scwrl_3BJ5.pdb: