Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------MESRLMKNKRYFFDTI---LIILLLISTIFCVSP---VFIK---------LDILGTPSHAILTFVLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIF-VIIIGARKYYRILFHQ--LMGFSPGIA---VFYKESQTTK-NLFKF---------YYFLYFTT-LISYYFFFTFVYDKPLLLPLISFSIIIALVQKLYRIENQQLFLLKSKVLTILESK-KNCE-FNLQDY--HEIWKLQ-SKSELPCVALS----YISLIKPYLSE-SVR-E---QIDLLEVKRFKK---INHPIS------------LYGMLDVIKLNL---YLRHYNEKNKYESMLKKILEVRPDFVLIEQNIDDSLNS------SQPLSLSLAISEIQLLLEVYMGIKHVSIRR---- 4C8H Chain:A ((474-925)) FRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGT----SLGYFRSFNQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSS--YGDPCIF---GSDNTLLLLSKWRSPE-------ESKWLPILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKEIQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKINNIREARYEQQ

General information: TITO was launched using: RESULT: Template: 4C8H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1756 -71609 -40.78 -228.05 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -40.78 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.271

(partial model without unconserved sides chains): PDB file : Tito_4C8H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C8H-query.scw PDB file : Tito_Scwrl_4C8H.pdb: