Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLGGGAISLVRNYIRNYGLGRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGDAITTIWANNDVNRRDPNLNALW 1Q90 Chain:M ((2-35)) --------------------------------------------------------------EAEFIAGTALTMVGMTLVGLAIGFVLLRVESLVE----------------------------

General information: TITO was launched using: RESULT: Template: 1Q90.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 4 -278 -69.50 -8.18 target 2D structure prediction score : 0.88 Monomeric hydrophicity matching model chain M : 0.52 3D Compatibility (PKB) : -69.50 2D Compatibility (Sec. Struct. Predict.) : 0.88 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.284

(partial model without unconserved sides chains): PDB file : Tito_1Q90.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q90-query.scw PDB file : Tito_Scwrl_1Q90.pdb: