Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLAILLITISETLPQ-VLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLLLVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILGGVLLGI--------------------------LSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQSSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFELSALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGF-ATVRFDWILIFSTLDAFFAGVLSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFG-TLAVSFVLLLFLLVAFVMLLNIRESI 3O7Q Chain:A ((30-431)) ------------LLCSLFFLWAVANNLNDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKL-SYKAGIITGLFLYALGAALFWPAAE--IMNYT---LFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHS----DAK----QGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEI-PGMT-AGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGH-VGLIALTLCSAFMSIQYPTIFSLGIKNLGQDT-KYGSSF-IVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARF-----

General information: TITO was launched using: RESULT: Template: 3O7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1718 -219795 -127.94 -589.26 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -127.94 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.235

(partial model without unconserved sides chains): PDB file : Tito_3O7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O7Q-query.scw PDB file : Tito_Scwrl_3O7Q.pdb: