Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------MLKRFLALVWLRCQII-----------------LSNKSILLQVLVPFAFTYFYKYL----METQGKVNDQQALVLLMMCLPFSL--------ALAVGSPITIILSEEKEKYNLQTLLLSGVK---GSEYILSTMF------------------------------------LPFLLTFV--IMGTTPLILGVTIVHT-------FNYITIVLLTSLSI---------ILFYLLIGLT-------AKSQVVAQVISLPAMILVAFLPMLSSLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLIWIVLLLTLITITIRKKKIS 2W0C Chain:L ((1-335)) MIVKKKLAAGEFAETFKNGNNITIIKAVGELVLRAYGADGGEGLRTIVRQGVSIKGMNYTSVMLHTEYAQEIEYWVGDLDYSFQEQTTKSRDVNSFQIPLRDGVRELLPEDASRNRASIKSPVDIWIGGENMTA-LNGIVDGGRKFEAGQEFQINTFGSVNYWVSDEEIRVFKEYSARAKYAQNEGRTALEANNVPFFDIDVPPELDGVPFSLKARVRHKSKGVDGLGDYTSISVKPAFYITEGDETTDTLIKYTSYGSTGSHSGYDFDDNTLDVMVTLSAGVHRVF-PVETELDYDAVQEVQHDW---YDESFTTFIEVYSDD---PLLTVKGYAQILMERT----------

General information: TITO was launched using: RESULT: Template: 2W0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 702 -66514 -94.75 -303.71 target 2D structure prediction score : 0.21 Monomeric hydrophicity matching model chain L : 0.64 3D Compatibility (PKB) : -94.75 2D Compatibility (Sec. Struct. Predict.) : 0.21 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.106

(partial model without unconserved sides chains): PDB file : Tito_2W0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0C-query.scw PDB file : Tito_Scwrl_2W0C.pdb: