Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKE-EGLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALLMEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREKDGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLF-NQMVDKVIVAGRDGVQISTSKKGK 3ENV Chain:A ((21-233)) -DEMKKAAGWAALKYVEKGSIVGVGTGSTVNHFIDALGTMSEEIKG----AVSSSVASTEKLEALGIKIFDCNEVASLDIYVDGADEINADREMIKGGGAALTREKIVAAIADKFICIVDGTKAVDVLGTFPLPVEVIPMARSYVARQLVKLGGDPCYREG----VITDNGNVILDVYGMKITNPKQLEDQINAIPGVVTVGLFAHRGADVVITGTPEGAKI-------

General information: TITO was launched using: RESULT: Template: 3ENV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1234 -3951 -3.20 -18.73 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -3.20 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_3ENV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ENV-query.scw PDB file : Tito_Scwrl_3ENV.pdb: