Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLMEHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWTSLEILTQTYQLGFYLNILLGILLIIKYFKVKQ 1I8N Chain:A ((37-125)) ----------------------ETITAGNEDCWSKRPG----------------WKLPDNLLTKTEFTSVDECRKMCEES---AVEPSCYILQINTETNECYRNNEGDVT--WSSLQ-YDQPNVVQWHLHACS-------------

General information: TITO was launched using: RESULT: Template: 1I8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 373 -52143 -139.79 -585.88 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -139.79 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.298

(partial model without unconserved sides chains): PDB file : Tito_1I8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I8N-query.scw PDB file : Tito_Scwrl_1I8N.pdb: