Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNGAVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYTGKLELLYDFYSAIGFCNEKLKLYLASDLTKVE-NPRPQDEDETLEVLEVSLEEAKELIQSGHI-CDAKTIMAVQYWELQKK 1V8R Chain:A ((11-164)) ---------RTYLYRGRILNLALE----------GRYEIVEHKPAVAVIALR-EGRMLFVRQMRPAVGLAPLEIPAGLIEPGED--PLEAARRELAEETGLSGDLTYLFSYFVSPGFTDEKTHVFLAENLKEVEA---------AIEVVWMRPEEALERHQRGEVEFSATGLVGVLYYH----

General information: TITO was launched using: RESULT: Template: 1V8R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 671 -59454 -88.60 -407.22 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -88.60 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.243

(partial model without unconserved sides chains): PDB file : Tito_1V8R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V8R-query.scw PDB file : Tito_Scwrl_1V8R.pdb: