Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MEESKEL-----NAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGIVDCNVLAMPAAIFFS----IENTNIS---------RMKRVTSSSYNIEKVCDVNQISRQLVG-----------------------------GQIDLETAFKQLTALQAQPLPYTKLQVTLAA------TFSAPFFSVMFSGNIYDALGAGV---ATLFGFAFSLYVEKFIR--------IPFVTAFAGAFVFGIIAQFWARY-TGFPSTADLIIAGAVMPFVPGIALTNAVRDIMTNHINS--GMSKMFESLLIT------LALGAGTSVALVLMN----------------------- 1JH8 Chain:A ((3-350)) TLHALLRDIPAPDAEAMARTQQHIDGLLKPPGSLGRLETLAVQLAGMPGLNGTPQVGEKAVLVMCADHGVWDEGVAVSPKIVTAIQAANMTRGTTGVCVLAAQAGAKVHVIDVGIDAEPIPGVVNMRVARGCGNIAVGPAMSRLQAEALLLEVSRYTCDLAQRGVTLFGVGELGMANTTPAAAMVSVFTGSDAKEVVGIGANLPPSRIDNKVDVVRRAIAINQPNPRDGIDVLSKVGGFDLVGMTGVMLGAARCGLPVLLDGFLSYSAALAACQIA-PAVRPYLIPSHFSAEKGARIALAHLSMEPYLHMAMRLGEGSGAALAMPIVEAACAMFHNMGELAASNIVLPE

General information: TITO was launched using: RESULT: Template: 1JH8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1265 -72375 -57.21 -288.34 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -57.21 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.312

(partial model without unconserved sides chains): PDB file : Tito_1JH8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JH8-query.scw PDB file : Tito_Scwrl_1JH8.pdb: