Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENAEDFAMLWDTYEDEVPHSIVH------ELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAGFKSFKLYADFEDKEPTETSTRWFFVAQK 3PX3 Chain:A ((46-230)) ------------------------------RHSPKAA---SLLDVACGTGMHLRHLADSFGTVEGLELSADMLAIARRRNPDA----VLHHGDMRDFSLGRRFSAVTCMFSSIGALAGQAELDAALERFAAHVLPDGVVV--VEPWWFPENFTPGYVAAGTVE----AGGTTVTRVSHSSREGEATRIEVHYLVAGPDRGITHHEESHRITLFTREQYERAFTAAGLS--------------------------

General information: TITO was launched using: RESULT: Template: 3PX3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 833 -65407 -78.52 -365.40 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -78.52 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.284

(partial model without unconserved sides chains): PDB file : Tito_3PX3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PX3-query.scw PDB file : Tito_Scwrl_3PX3.pdb: