Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRTRMSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSGIDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVLACLREPLPASHYMIAVAGKD-VRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAGAQNLLNAFNIVEEVEYCAKIYCLAKNFGEPV-VLPDEEMELMAEKFKTYGQRK 1E4B Chain:P ((1-206)) MERNKLA---RQIIDTCLEMTRLGLNQGTAGQVSV--RYQDGMLITPTGIPYEKLTESHIVFIDGNGKHEEG-KLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVSILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIACEVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKF-------

General information: TITO was launched using: RESULT: Template: 1E4B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1100 -81990 -74.54 -401.91 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain P : 0.83 3D Compatibility (PKB) : -74.54 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_1E4B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E4B-query.scw PDB file : Tito_Scwrl_1E4B.pdb: