Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVGKIELFVDRPE--GEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDE--GHGETEDILAVIEHVRKLHAGLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVG-L-----------------R-HY----------------K--TP--EIQGDILLIHGEQDDITLLSDAIEWAKP-QKHPITILSGANHFFTGYLKQLRQIITRFIIMK 2WTN Chain:B ((8-247)) ---------------CDGIKLNAYLDMPKNNPEKCPLCIIIHGFTGHSE-ER--HIVAVQETLNEIGVATLRADMYGHGKSDGKFEDHTLFKWLTNILAVVDYAKKLDFVTDIYMAGHSQGGLSVMLAAAMERD--IIKALIPLSPAAMIPEIARTGELLGLKFDPENIPDELDAWDGRKLKGNYVRVAQTIRVEDFVDKYTKPVLIVHGDQDEAVPYEASVAFSKQYKNCKLVTIPGDTHCYDHHLELVTEAVKEFMLE-

General information: TITO was launched using: RESULT: Template: 2WTN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1050 -51073 -48.64 -260.58 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -48.64 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2WTN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WTN-query.scw PDB file : Tito_Scwrl_2WTN.pdb: