Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIYLNGELTD-T-PSQNLLQLIQELALEGKRFAVEHNQQIVPKSKLEQISIAQHDRIEIIHAVGGG 1RYJ Chain:A ((11-73)) TVITDDGKKILESGAPRRIKDVLGELEIPIETVVVKKNGQIVI----DEEEIFDGDIIEVIRVIYGG

General information: TITO was launched using: RESULT: Template: 1RYJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 193 5256 27.23 86.16 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 27.23 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_1RYJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RYJ-query.scw PDB file : Tito_Scwrl_1RYJ.pdb: