Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRGYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLRKR----GIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 3G82 Chain:C ((128-219)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------VDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDV-IKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNF------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3G82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 145 -18061 -124.56 -205.24 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain C : 0.53 3D Compatibility (PKB) : -124.56 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_3G82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G82-query.scw PDB file : Tito_Scwrl_3G82.pdb: