Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MPIDTQLTTLFCLIDDFCTDITQNVEQ------YMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSY----SRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTK--LAVCHN------KRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKL--FGDRGYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLKKRGIIEAPFKLMKSQFDLEHSRHRSKMGLL-----------TTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA------------------------ 4D08 Chain:A ((4-342)) DDEYTKLLHDGIQPVAAIDSNFASFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSRKDYQRMLDLMRDIILA-------------TDLAHHLRIFKDLQKMAEVGYDRNN-KQHHRLLLCLLMTSCD-------LSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDREKAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPSNNSLDFLD

General information: TITO was launched using: RESULT: Template: 4D08.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1213 -30195 -24.89 -107.84 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -24.89 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_4D08.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D08-query.scw PDB file : Tito_Scwrl_4D08.pdb: