Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEQVMVE-----LRLIEQ---TFRLATTSDKREELERAAELLNEKFNDMRRSAPRVEHNKLVIMVA--------LQLTQEVLSLNKSLQE-YAHCE----RLLQTILEDVEQIV-- 1P57 Chain:A ((5-114)) PLYPVQVSSADARLMVFDKTEGTWRLLCSSRS---NARVAGLSCEEMGFLR----ALTHSELDVRTAGAAGTSGFFCVDEGRLPHTQRLLEVISVCDCPRGRFLAAICQDCGRRKLP

General information: TITO was launched using: RESULT: Template: 1P57.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 332 4941 14.88 56.79 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 14.88 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_1P57.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P57-query.scw PDB file : Tito_Scwrl_1P57.pdb: