Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTPEDISQNKPLLWLMAAACGLCAGVNYYCQPLIHSIQQD------FAVTQAQAALTVTFAQVSYALGLLFIVPMGDI-VNKAKAIPFLMMLCAVGLFTSAFAVNLPMLWIGTILAGLFS-VAAQVLIPLATMTVKP--EKTGEIIGFLMSGLLVGILLSTSLAGLFSNLFHWKVVYAISGILMLLLAYTLKSRLPYVMRM-------KLN--YGQIF-------------------------------------------------VSMA---QLLKEEKRLLLRALTGAFAFASVSILYSTIALLMTSAHHLP----DLFIGLV-PLVGIFGALSTRYIGKYADQGYTRL-----LTWMGCGLFLLSWICFYFGQT-------LLSAYVIGFALIQLALALVHTSNQSIIFR-LRPDAKSRINAIYMTTYFIGGAAGSALGIFAWNRGEWTMTCLAGVGLVVFCILFSMIDAFLQKKQMA 4IKV Chain:A ((23-477)) -----------KGLFTLFFTEFWERFSYYGMRAILVYYMYYEVSKGGLGLDEHLALAIMSIYGALVYMSGIIGGWLADRVFGTSRAVFYGGLLIMAGHIALAIPGGVAALFVSMALIVLGTGLLKPNVSSIVGDMYKPGDDRRDAGFSIFYMGINLGAFLAPLVVGTAGMKYNFHLGFGLAAVGMFLGLVVFVATRKKNLGLAGTYVPNPLTPAEKKKAAAIMAVGAVVIAVLLAILIPNGWFTVETFISLVGILGIIIPIIYFVVMYRSPKTTAEERSRVIAYIPLFVASAMFWAIQEQGSTILANYADKRTQLDVAGIHLSPAWFQSLNPLFIIILAPVFAWMWVKLGKRQPTIPQKFALGLLFAGLSFIVILVPGHLSGGGLVHPIWLVLSYFIVVLGELCLSPVGLSATTKLAPAAFSAQTMSLWFLSNAAAQAINAQLVRFYTPE-NETAYFGTIGGAALVL----------------

General information: TITO was launched using: RESULT: Template: 4IKV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1417 -188144 -132.78 -514.05 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -132.78 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_4IKV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IKV-query.scw PDB file : Tito_Scwrl_4IKV.pdb: