Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MMLT------------SKASLRLTLLASAIFLVACQPKSDPKESE-DQQK---PAVVEQKPVELTLKGETVPSKVTLPDCDGKTCPEFTVARLQSNFPFIDKIIDQQVLKALGQILEIAEPDAKAAQADKKTEA-S-TAAATEQ-QD---SFDAQVQRYANSFIDLDNDLKALSSNHQINLLVKPKIIQ-SQGKV-VTVVVNSSSYLGGAHGSAAQQYYNFD---LKKEKQVKLEGLLRPEKKAALEKLAHEAFKAWVTDSKLV-NSVSEYEQAWPFKLTENFLLGDQGLIL--QYGEYEIGPYVVGLPR-LVIPYDQLQDVLKEEYLPQPKAKPASTPAVKSTS 3DSM Chain:A ((1-327)) ASGLFITNEGNFQYSNATLSYYDPATCEVENEVFYRANGFKLGDVAQSMVIRDGIGWIVVNNSHVIFAIDINTFKEVGRITGFTSPRYIHFLS----DEKAYVTQIWDYRIFIIN--------PKTYEITGYIECPDMDMESGSTEQMVQYGKYVYVNCWSYQNRILKIDTETDKVVDELTIG--IQPTSLVMDKYNKMWTIT--DGGYEGSPYGYEAPSLYRIDAETFTVEKQFKFKLGDWPS---EVQLNGTRDTLYWINNDIWRMPVEADRVPVRPFLEFRD--TKYYGLTVNPNNGEVYVADAIDYQQQGIVYRYSPQGKLIDEFYVGIIPGAFCWKLEHHHHH

General information: TITO was launched using: RESULT: Template: 3DSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1631 80055 49.08 273.22 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 49.08 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.025

(partial model without unconserved sides chains): PDB file : Tito_3DSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DSM-query.scw PDB file : Tito_Scwrl_3DSM.pdb: