Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTQHSALNARKQQATPRGVGVMCQWYAEKAENATIWDKEGNQFIDFAGGIAVLNTGH-RHPKVIAAVTEQLTKFTHTA----YQVTPYESYVALAERINERAPIAGPAKAAFFTTGAEAVENAVKIARCYTGRHGIITFGNGFHGRSFMTMAMTGKTAPYKRDFGVMPAGVFHARYPVPAKGIS-----------VDAAIESVED-IFSEDIAPHDVAVIVLEPVQGEGGFNVVPAEFLKRLRAICDKHGILLVADEVQSGFARTGKLFAMNHYETKADLITMAKSLGGG-FPISGVVGRAEVMDAPNPGGLGGTYAGSPIAVAAAHAVIDAIEEENLCDRANELGAELVATLKDIQQATGDVVTDIRALGSMVAVELETAEQAKVVQNYAMENGLLLLTCGKYGNVIRFLYPLTIPAEQFRQGLDILKQGFATLKAGSAKAMEQSA 2EO5 Chain:A ((29-418)) ----------------------------DHGEGVWIYDVDGNKYLDFTSGIGVNNLGWPSHPEVIKIGIEQMQKLAHAAANDFYNIPQLE----LAKKLVTYSPGNFQKKVFFSNSGTEAIEASIKVVKN-TGRKYIIAFLGGFHGRTFGSISLTASKAVQRSIVGPFMPGVIHVPYPNPYRNPWHINGYENPSELVNRVIEFIEDYIFVNLVPPEEVAGIFFEPIQGEGGYVIPPKNFFAELQKLAKKYGILLVDDEVQMGLGRTGKLFAIENFNTVPDVITLAKALGGGIMPIGATIFRKD-LDF-K------TFGGNALACAIGSKVIDIVKD--LLPHVNEIGKIFAEEL----QGLAD---DVRGIGLAWGLEYNEKKVRDRIIGESFKRGLLLLPAGR--SAIRVIPPLVISEEEAKQGLDILKKVIKVV------------

General information: TITO was launched using: RESULT: Template: 2EO5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2185 7373 3.37 20.14 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 3.37 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_2EO5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EO5-query.scw PDB file : Tito_Scwrl_2EO5.pdb: