Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMAEEVHHAPNLKRSKYFATMALIIVVVLWIGLLITNRFLPEYTDLIHILMLGAEAGVVGGLADWYAITILFRNPFGKLPIPKFLRDHTEIIPRNKARIAESMGRFVQENFLSPQVVERSLEKTDLSLAIGQWLASPQNNTQVVQLIQQTVPKVFEFVSQEQIANFVQNNSVQWVRNTQVNKLASEMLRAVLEN----DFHQDVLQRGLDIAHEW-VVQ--NPDKTRELTRKMFKALGVWSLAKGASWIGIDVQQRTIDSLVEKVESMLAD-HEHPWRQEIETIAHSL-MLELANPESIASQRLNSGKDALLDSPQVLNFISGAVTILCDAIKEDLMKEDSGIAMNLRAAIQQLGENLVQNVKVREVLNKEMTGLAMNFTDQYSHKIIRYVSERIHEWDSREMIGKIENEVGGDLHMIRVNGVVVGAFIGLTLGVIRAAIESIL 3ZTA Chain:A ((8-146)) --------------------------------------------------------------------------------------------------------------------------------------------------KAENIVEKAINLLSKEDQAG---------VHFNEISALTRDFCRAILSDLEQSGFTTSELEKEIADKVKIMFAQGYHIEVLQLILEKILDSFISVIREQYHDLQAAASYITTVRDHIFKGTSFLLKMALQTQREVIQKQNEALMELST-------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZTA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 -98 -97.50 -0.75 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -97.50 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.53 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_3ZTA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZTA-query.scw PDB file : Tito_Scwrl_3ZTA.pdb: