Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNVLKHFKEKQMPSFQSADAQINYQTFGEPSSPALVFSNSLGTNYGMWQKQFNELKDQFFVICYDNRGHGSS-STPDGPYTVEQLGEDVVRLLDHLNISKAAFCGISMGGLTGQWLAIHYPNRFSHVVVANTAAKI-----------------GQEQAWLDRAKLVREQGLQP---IATTAASRWFTDPFIQSHPSIVNNLCNDLSAGSAMGYANCCEALAKADVREQLKDIKIPVLVIAGPQDPVTTVADGEFMQQRIPQS-KLAEIDASHISNVEQPEVFNKILKDFLVN 3V48 Chain:A ((15-250)) --------------------------------APVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLF----LYPADWMAARAPRLEAEDALALAHFQGKNNLLRRLN------------ALKRADFSHHADRIRCPVQIICASDDLLVPTACSSELHAALPDSQKMVMPYGGHACNVTDPETFN---------

General information: TITO was launched using: RESULT: Template: 3V48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1047 -67471 -64.44 -315.29 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -64.44 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_3V48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V48-query.scw PDB file : Tito_Scwrl_3V48.pdb: