Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFTGMSNQRIRIRLKSFDHRLIDQSAQEIVETAKRTGAQVCGPIPMPTRIERFNVLTSPHVNKDARDQYEIRTYKRLIDIVQPTDKTVDALMKLDLAAGVDVQIALG 3IMQ Chain:J ((6-87)) -----MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTR---------------------SRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISLG

General information: TITO was launched using: RESULT: Template: 3IMQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 330 -43055 -130.47 -525.06 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain J : 0.86 3D Compatibility (PKB) : -130.47 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_3IMQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IMQ-query.scw PDB file : Tito_Scwrl_3IMQ.pdb: