Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MECSMNLADRDGFIWQDGQLIDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKGTAIFRLQDHTKRLLNSAKIYQMKVPYDQAALEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATN-NTIHAAVAAWAWGAYLGDEAMAKGIR-----VKTSSFTHHHPNVTMCKAKASGNYTLSILAHQEVAHSGYDEAMLMDPQGYVCQGSGENVFLIKDGVLHTPDIAGGALDGITRQTVMTIAKDLGYEVVERRITRDEFYIADEAFFTGTAAEVTPIREYDDRQIGAGCRGPITTEIQKTFFDAVQGKKPKYEHWLTYVK 5CM0 Chain:A ((5-279)) ------------LVYMNGEFVPESQAKVSVFDHGFLYGDGVFEGIRAYNG----KVFKLYEHIDRLYDCARVIDLKIPLSKEEFAEAILETLRRNNLRDAYIRPIVTRGAGDLGLDPRKCPSPNVIIITKPW---------EKGLKAITVAIRRNAIDSLPPNI------KSLNYLNNILAKIEANAKGGDEAIFLDHNGYISEGSGDNIFIVKNGTITTPPTLNN-LKGITRQVVIELINELEIPFREANIGLFDLYSADEIFVTGTAAEIAPVTYIDGRTVGNGKPGKVTKMLMEKF-------------------

General information: TITO was launched using: RESULT: Template: 5CM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1361 -46466 -34.14 -178.03 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -34.14 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_5CM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CM0-query.scw PDB file : Tito_Scwrl_5CM0.pdb: