Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMTESRLTHLKQLEAESIHIIREVAAEFE-NPVMLYSIGKDSAVMLHLALKAFYPAKLPFPLLHVDTGWKFKDMIAFRDNMAKTHGFDLIVHQNKEGRE----A--GINPF-DHGSSKYTDIMKTQALKQALDKYQFDAAFGGARRDEEK-SRAKERVYSFRDSKHRWDPKNQRPELWNLYNGKVNKGESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEVPQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHDEAGSMEKKKQEGYF 2GOY Chain:D ((41-249)) ----------------SPQDILKAAFEHFGDELWISFSGAEDV-VLVDMAWKLN----RNVKVFSLDTGRLHPETYRFIDQVREHYGIAIDVLSPDPRLLEPLVKEKGLFSFYRDGHGECCGIRKIEPLKRKLA--GVRAWATGQRRDQSPGTRSQVAVLEIDG----A------------F--STPEKPLYKFNPLSSMTSEEVWGYIRMLELPYNSLHER---------------------------------GYISIGCEPCTRPVLPN---------------QHEREGRWWWE---------------

General information: TITO was launched using: RESULT: Template: 2GOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 917 17513 19.10 87.56 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : 19.10 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_2GOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GOY-query.scw PDB file : Tito_Scwrl_2GOY.pdb: