Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEFLKAYVQTFKDIVSNSSAFTTLFLSVILYSFFYPTAYKAERAESIPIVIVDEEQSLLTSQVIGQTANSPHVKIVDVTANFLEAEQMVREQKADGILLLPSNLTQSLRRGETGGIGLYLSTTNFLKTKEIGLGLATSIEATLKEYIERFGQRTHFQPALSIHQMPLFNTLSGYGSYIFPAVASLIIHQTIVLGLAMLVASYREQHEKITPIRFAGIFASIFTIGCLGSFYLFGFTLWFNDYPH-GGNFVGLLVAVPIFISCVIGLGMLIGSLLDILERAGHIIVFSSVPLFLL-TGAAWPHQAMPEWLQWFAWCLPSTHAVQMFVQLNQMGVPLN-----------------------VVAPKLIFLATIGVIFLITAYSRLKVSK 5DO7 Chain:C ((461-645)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WQMMLAYALHVLPFSVVATMIFSSVCYWTLGLHPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIISYFTFQKYCSEILVVNEFYGLNFTCGSMCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIR-

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 474 -85361 -180.09 -565.30 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.56 3D Compatibility (PKB) : -180.09 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: