Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQRQLVSSSRVEQKNWDR-----------PVLSEWIEQFVNTVQAVQ-TPVQIVAHSFGCLTSVAALAEHPELQLQVKKLILVAPANPARFGEA------------------------GFAR---------HS-LTDYK---------------D-----YFHQ--LKINVPATLLISENDPWLDFFDALQLAKSW-Q-LTPINLGQVGHINVASGFGPFPDLLNHLLPEDSMCSHSSIDPVKTHFSLKFA 3W04 Chain:B ((45-264)) ------RVVLYDLVCAGSVNPDHFDFRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRRP---DLFAKLVLIGASPRFLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADVPAAVQEFSRTLFNMRPDISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVAAYLKAHLGGRTTVEFLQTEGHLPHLSAPSLLAQVLRRALA----------------------

General information: TITO was launched using: RESULT: Template: 3W04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 494 -30392 -61.52 -202.61 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -61.52 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3W04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W04-query.scw PDB file : Tito_Scwrl_3W04.pdb: