Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------MKNKNIGCWLLLAGSSSVCAMQPLDDQSLAAATGQNGLTLGIQADQVKFNQVALIDTNGIASTSYNSKAGLIIAGNSTNPVPGIEFIKAAVSTNPSFNIAIDTDAGGGNPFLNLAVTMGSD--VNGIRLLPFSVYLAPSTSLPSPSDYALTSYAPKS------------IFSSGTTV---------NTGVKELIRST----------------GNLDINFVQTNKPRLNIQLGHAAQSVMVKFGGAIQSIC--SAASGCPITLVSD-------------NTGATFGFKFA---GTNASTGFVLDGFYAGVDPTGLTIGNIGVSSKFDAS--------LNNVTLGNLGTQ----------STTTFNNLPNGSMGSFGVTGASVTDFKMKVSGF----------------------- 5FN0 Chain:A ((6-460)) NARQVTIIGAGLAGTLVARLLARNGWQVNLFERRPDPRIETGARGRSI-NLALAERGAHALRLAGLEREVLAEAVMMRGRMVHVPGTPPNLQPYGRDDSEVIWSIN-RDRLNRILLDGAEAAGASIHFNLGLDSVDFARQRLTLSNVSGERLEKRFHLLIGADGCNSAVRQAMASVVDLGEHLETQPHGYKELQITPEASAQFNLEPNALHIWPHGDYMCIALPNLDRSFTVTLFLHHQSPAAQPASPSFAQLVDGHAARRFFQRQFPDLSPMLDSLEQDFEHHPTGKLATLRLTTWHVGGQAVLLGDAAHPMVPFHGQGMNCALEDAVALAEHLQSAADNASALAAFTAQRQPDALAIQAMALENYVEMSSKVASPTYLLERELGQIMAQRQPTRFIPRYSMVTFSRLPYAQAMARGQIQEQLLKFAVANHSDLTSINLDAVEHEVTRCLPPLSHL

General information: TITO was launched using: RESULT: Template: 5FN0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1640 -10231 -6.24 -32.48 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.24 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.022

(partial model without unconserved sides chains): PDB file : Tito_5FN0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FN0-query.scw PDB file : Tito_Scwrl_5FN0.pdb: