Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWKMDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVN-EHRNLQLSDNERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVLLGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTT------HAFIEKVIFPKVKAGAFSHEMDHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQAAAENALAAL 2EZ6 Chain:A ((3-217)) ---MLEQLEKKL----GYTFKDKSLLEKALTHVSYSKKEH-------YETLEFLGNALVNFFIVDLLVQYSPNKREGFLSPLKAYLISEEFFNLLAQKLELHKFIRIKRGKINE------TIIGDVFEALWAAVYIDSGRDANFTRELFYKLFKEDILSAIKEGRVKK--DYKTILQEITQKRWKERPEYRLISVEGPHHKKKFIVEAKIKEYRT-LGEGKSKKEAEQRAAEELIKLL

General information: TITO was launched using: RESULT: Template: 2EZ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 822 8097 9.85 38.93 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 9.85 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2EZ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EZ6-query.scw PDB file : Tito_Scwrl_2EZ6.pdb: