Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------------------------------------------------MFRRYPYSDSSNSEEK------RRKNMKYFQLVLTLITAMVIEVLEQKLA--------------------------------------------------------------------------------------- 4D10 Chain:E ((24-333)) SIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINI

General information: TITO was launched using: RESULT: Template: 4D10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 27 -167 -6.19 -3.80 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain E : 0.52 3D Compatibility (PKB) : -6.19 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_4D10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D10-query.scw PDB file : Tito_Scwrl_4D10.pdb: