Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVLSI---SKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPG------EITDNNGILTFNFSDPEGNYFVIGKAESSD 2P25 Chain:A ((8-119)) -----------------------HVAINASNYQATKNFYVEKLGFEVLRENHRPEK-NDIKLDLKLGSQELEIFISDQFPARPSYPEALG-LRHLAFKVEHIEEVIAFLNEQGIETEPLRVDDFTGKKMTFFFDPDG------------

General information: TITO was launched using: RESULT: Template: 2P25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 277 -14911 -53.83 -144.77 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -53.83 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_2P25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P25-query.scw PDB file : Tito_Scwrl_2P25.pdb: