Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEELAGEHRLFEKRNCDKTF-----VTVPSFFCYAILDVKNRRNKM-----REIYSFESKIYA----SEVGKGGAYIIFPYD-IRKEFGAGRVKVQATFDNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK 1U88 Chain:A ((131-209)) ---------KMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQAT--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1U88.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 160 -285 -1.78 -4.45 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -1.78 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.092

(partial model without unconserved sides chains): PDB file : Tito_1U88.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U88-query.scw PDB file : Tito_Scwrl_1U88.pdb: