Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKENFKRSVSRMNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYKDALAMSESGQVIRTYPMTPGIDLSGTVVQSNNP---------RFSKEDLVLATGF----------------GLGVTHPGGYSQYQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGVGSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQID----YVIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPIDQRLSLWQQMADLQIADELVVQEIT----LDQLPETASKLLAGTHQGRTLVNVGDHK 3PII Chain:A ((10-337)) ----------------------KEPL---KIKEVEKPTISYGEVLVRIKACGVCHTDLHAAHGDWPVKPKLPLIPGHEGVG-IVEEVGPGVTHLKVGDRVGIPWLYSACGHCDYCLSGQETLCEHQKNAGYSVDGGYAEYCRAAADYVVKIPDNLSFEEAAPIFCAGVTTY---KALKVTGAKPGEWVAIYGI-GGFGHVAVQYAKAMGL-NVVAVDIGDEKLELAKELGADLVVNP---LKEDAAKFMKEKVGGVHAAVVTAVSKPAFQSAYNSIRRGGACVLVG------LPPEEMPIPIFDTVLNGIKIIGSIVGTRKDLQEA---LQFAAEGKVKTIIEVQPLEKINEVFDRMLKGQINGRVVLTLE---

General information: TITO was launched using: RESULT: Template: 3PII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1719 -16977 -9.88 -57.55 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -9.88 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_3PII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PII-query.scw PDB file : Tito_Scwrl_3PII.pdb: