Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHKGIYAGLLG---TVILFSACGYKTKNSVKESSAAISTTEVGSTKSTSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPS--LPLENSDKALLFLIAHRSELQ-SDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTPF-- 1JSD Chain:B ((1-160)) GLFGAIAGFIEGGWPGLVAGWYGFQHSND--QGVGMAADSDSTQKAIDKITSKVNNIVDKMNKQYGIIDHEFSEIETRLNMINNKIDDQIQDIWTYNAELLVLLENQKTLDEHDANVNNLYNKVKRALGSNAMED------GKGCFELYHKCDDQCMETIR-NGTYNRR

General information: TITO was launched using: RESULT: Template: 1JSD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 376 19098 50.79 125.64 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : 50.79 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.152

(partial model without unconserved sides chains): PDB file : Tito_1JSD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JSD-query.scw PDB file : Tito_Scwrl_1JSD.pdb: