Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVSYLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEK-RKVPRLYSIIG----LFVLVVALIAWGSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEI-LRDPLFNQFREQLETAGDKFMNSAGDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDG------------KQLAPYFVKFLPTRMQKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLIPYLG---SFLAMIPAIFLAIVAGPF----------MIIKVLIVFAVEQTIEGR--LISPLVLGNQLAIHPVTILL----------VLLTSGKLFG---------------IIGVILGIPVYAAAKVVIT-------HVFEWY-----TTISSLYEENPLKKQKE------------------- 5F0J Chain:A ((4-461)) GSKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLS--------PKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVG-VVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEP--------------TDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQ

General information: TITO was launched using: RESULT: Template: 5F0J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1558 -104649 -67.17 -286.71 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -67.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.247

(partial model without unconserved sides chains): PDB file : Tito_5F0J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F0J-query.scw PDB file : Tito_Scwrl_5F0J.pdb: