Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAASRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRGDISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQNTTDPLNMTPGTDPMDVPGETDTM----GTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVTTGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQVDVSKHTVIREEVGIH--KEEHEDVEKVTEDVSREELDI-DTSGDVHIEDRNKKS 3PM6 Chain:A ((10-302)) LKSNRALPLLTFARTHSFAIPAICVYNLEGILAIIRAAEH--KRSPAMILLFPWAIQYADSLLVRTAASACRAA------------SVPITLHLDHA-------QDPEIIKRAADLSPGFDSIMVDMSHFS-KEENLRLTRELVAYCNARGIATEAEPGRIEGGEDGVQDTVDL-EGVLTTPEESEEFVATGINWLAPAFGNVHGNYGPRGVQLDYERLQRINEAVGERVGLVLHG------------------------------ADPFTKEIFEKCIERGVAKVNVN-RAVNNEYVKVMREKAGSLPITRLHEEVTNAMQAAVEKIMDMIDSTGKAEFM------

General information: TITO was launched using: RESULT: Template: 3PM6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1520 119693 78.75 446.61 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 78.75 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_3PM6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PM6-query.scw PDB file : Tito_Scwrl_3PM6.pdb: