Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPS-IEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQN-HDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTPQTFRCKDFMDLYGSLSDEE-KEILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD 3Q80 Chain:B ((7-230)) EVVAIVPAAGSGERLA-VGVPKAFYQLDGQTLIERAVDGLLDS-GVVDTVVVAVPADRTDEARQILGHR-------AMIVAGGSNRTDTVNLALTVL-------EPEFVLVHDAARALTPPALVARVVEALRDGYAAVVPVLPLSDTIKAVDANGVVLGTPERAGLRAVQTPQGFTTDLLLRSYQR----LPAAEYTDDASLVEHIGGQVQVVDGDPLAFKITTKLDLLLAQAIVR--

General information: TITO was launched using: RESULT: Template: 3Q80.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1135 -51054 -44.98 -239.69 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -44.98 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3Q80.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q80-query.scw PDB file : Tito_Scwrl_3Q80.pdb: