Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV--IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKAKKNYERLGLTDKVTLLEGQAADILP--ELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGGVLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTIL-PLG----DGVILMTKESETIEL 3TFW Chain:B ((6-224)) -------MQQQWSAVDNYLIKALIPGDPVLDRVLENNHRAGLPAHDVAANQGQFLALLVRLTQAKRILEIGTLGGYSTIWMARELPADGQLLTLEADAHHAQVARENLQLAGVDQRVTLREGPALQSLESLGECPAFDLIFIDADKPNNPHYLRWALRYSRPGTLIIGDNVVRDGEVVNPQS-----ADERVQGVRQFIEMMGAEPRLTATALQTVGTKGWDGFTLAWVNA-----

General information: TITO was launched using: RESULT: Template: 3TFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1007 6875 6.83 32.74 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : 6.83 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_3TFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TFW-query.scw PDB file : Tito_Scwrl_3TFW.pdb: