Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTESTTPKFAIPELPMQIQTIRQYLPHRYPFLLVDRVTEVTDN-SIIGYKNVSINEEFLQGHFPEYPIMPGVLIVEALAQVSGVLGFI----MNNETPKPGSLFLFAGAERVRFKKQVVAGDQLVLKSELVMQKRGIYKYNCTASVDGIVAATAEIMISHQKTEQA 3D04 Chain:F ((14-151)) -----------------IEHILQILPHRYPMLLVDRITELQANQKIVAYKNITFNEDVFNGHFPNKPIFPGVLIVEGMAQSGGFLAFTSLWGFDPEIAKTKIVY-FMTIDKVKFRIPVTPGDRLEYHLEVLKHKGMIWQVGGTAQVDGKVVAEAEL----------

General information: TITO was launched using: RESULT: Template: 3D04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 563 -45679 -81.13 -343.45 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain F : 0.80 3D Compatibility (PKB) : -81.13 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_3D04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D04-query.scw PDB file : Tito_Scwrl_3D04.pdb: