Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDIQGIKEALPHRYPMLLVDRVLEV-SEDTIVAIKNVTINEPFFNGHFPQYPVMPGVLIMEALAQTAGVLELSK-----PE-NKGKLVFYAGMDKVKFKKQVVPGDQLVMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN 3D04 Chain:B ((14-151)) ---IEHILQILPHRYPMLLVDRITELQANQKIVAYKNITFNEDVFNGHFPNKPIFPGVLIVEGMAQSGGFLAFTSLWGFDPEIAKTKIVYFMTIDKVKFRIPVTPGDRLEYHLEVLKHKGMIWQVGGTAQVDGKVVAEAEL------

General information: TITO was launched using: RESULT: Template: 3D04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 535 -22758 -42.54 -173.73 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -42.54 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_3D04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D04-query.scw PDB file : Tito_Scwrl_3D04.pdb: