Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGR--------------STGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAF-SVFPVGSKVLAVRDLYGGSFRWFNQVEQE--GRFHFTY-ANTEEELIA----ELE-KDVDVLYIETPTNPLMLEFDIEKLAKLAHAK------GAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYT------------------GRGGMISFKVADE-TRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG 5IJG Chain:B ((14-426)) --HLLHPETQMLNYGYDSELSEGAVKPPVFLTSTFVFKTAEDGRDFFDFVSGRKEP---LVYSRFNHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVRPGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEATIEKAAEEALAKGRVSVILIETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLTKYVGGHSDLIAGAVLGA-KSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSNARAIAEFLRNHPKVEKLHYLPFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLNALQILKLAV---GTESLASHPAA-------VDVRERIGVLESTIRLSIGIEHPDDLIADLAQALDA

General information: TITO was launched using: RESULT: Template: 5IJG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1753 -21181 -12.08 -60.86 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -12.08 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_5IJG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IJG-query.scw PDB file : Tito_Scwrl_5IJG.pdb: