Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFRAMANKKLLICAAIAAGLLLTACVKKETPKEEEQDKVETAVSEPQPQKPAKFESLESVDTQETQVQEQPQVEVHREETANTTTEIRRETRPARSDESSQTQVAEQPKSETPKVEPKPEKKPEPKAEPKPEKAQSKPAAKATEPANTEDDAVAAAIAAATPALKN 5E6F Chain:A ((2-145)) ---------TIICSVDIGIKNPAYTIFRYEDSKVSLIAIEKSDWSDNWEYNVTKDLTKYNPD----------IIVLEKQGYRSPNAKIIYFIKGFFYNTNTSVIVRN------------------PTFQGGSYSDRKKQSVITFMDKLSKLDDIADSFNLGIAYIES

General information: TITO was launched using: RESULT: Template: 5E6F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 586 -29749 -50.77 -228.83 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -50.77 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_5E6F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E6F-query.scw PDB file : Tito_Scwrl_5E6F.pdb: