Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGHVENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDT--GGRYIVICYKATEFSGTL-QSSEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF 4HFQ Chain:B ((67-194)) -----TPLMDVRAWIVE--DEKICLVRGQ----GEDSWALPGGFGEVGYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQENQEIADLQFFAIDQLPNLSEKRITKEQIELLWQVY-------------------

General information: TITO was launched using: RESULT: Template: 4HFQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 620 -37767 -60.91 -302.14 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -60.91 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_4HFQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HFQ-query.scw PDB file : Tito_Scwrl_4HFQ.pdb: