Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MLFRAMANKKLLICAAIAAGLLLTACVKKETPKEEEQDKVETAVSEPQPQKPAKFESLESVDTQETQVQE-QPQVEVHREETANTTTEIRRETRPARSD-ESSQTQVAEQPKSETPKVEPKPEKKPEPKAE--PKPEKAQSKPAAKATEPANTEDDAVAAAIAAATPA-LKN 5TOD Chain:A ((18-182)) LASLFAFKSFRENWQRAWVRALNEQACIQIAF--------------EEVPQLPPR-ASISHVTCVDQSEHTMVLRCQLSAEEVRFPVSVTQQS--PAAVSMETYHVTLTLPPTQLEVNLEEIPGEGLLISWAFTDRPDLSLTVLPKL---ELSTIEELIKDAIVSTQPAMV--

General information: TITO was launched using: RESULT: Template: 5TOD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 557 62203 111.68 431.97 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 111.68 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.149

(partial model without unconserved sides chains): PDB file : Tito_5TOD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TOD-query.scw PDB file : Tito_Scwrl_5TOD.pdb: