Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSASVNNLDGKLDLVDITALSAAYEPEEVLGEGTYGIVFRARNKATGAKYAIKKLRLDGFSEGVPATTIREATLLHDLNDNPNVVKLLDVVCSDHRVYLVFELLDEDLRSFIKRYRPVSGQKPTNGTAVPLHLVREFTRQILYALWTCHNNRILHRDLKPGNILVANYRSKNGETKFYVKLADFGLARMFEMSVQTYTHEVMTLWYRSPEIILGDRHYTPAADVWSVGCIVAEMILGYSLFRGENWRDQLDKVFYVVGTPTEQTWPGVTKLPGYDRNFKVYHVAPLPTRLRDYDEKAVEFIAYLLVTNPKLRPTIPEILEHPFMKDA 1GIH Chain:A ((4-288)) -----------------------FQKVEKIGEGTYGVVYKARNKLTGEVVALKKIR--------PSTAIREISLLKELN-HPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMD---------ASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLI--------NTEGAIKLADFGLA--------------VTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 1GIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1237 -179745 -145.31 -683.44 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -145.31 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_1GIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GIH-query.scw PDB file : Tito_Scwrl_1GIH.pdb: