Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNL---STSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSQDNGMMESFFGILKFMAMRKHLNHLINWNKLL 3OYD Chain:A ((136-249)) ------------LPPSQGYLYVLVVVDGMTGFTWLYPTKAPSTSATVKSLNVLTSIAIPK------VIHSDQGAAFTSSTFAEWAKERGIHLEFSTPYHPQSSGKVERKNSDIKRLLTKLLVGRPTKWYDLL

General information: TITO was launched using: RESULT: Template: 3OYD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 375 -3991 -10.64 -35.95 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -10.64 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.296

(partial model without unconserved sides chains): PDB file : Tito_3OYD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OYD-query.scw PDB file : Tito_Scwrl_3OYD.pdb: