Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNHENYWTFCPHNLFWIDRKINRIDMSQTTQTDIITLGDVATRLPSFIPKVPHILNGLKQAYLRTSNTPTGLGIAFEKAVKRNPKGTALLFE------DQSYSYEALNEWANQISHYYLSLGARKGDVIAVMIENRSELIATIVGLAKIGVTIALV-----NTSQVGKVLAHSINLVKPIAVIAGEEVRAA--------IDEIRQDLNVPKDRFHWFADQATRQKSGTAPKGYVNLADQIDQF-PKFNPSTTRSVTGNDGLFYIYTSGTTGLPKAVIFKHSRWTL-AYGTYGHILNLGPDDVMYVTLPLYHATGVVVCWCGVIAGSATLAIRR---KYSTSA-FWKDVQKFNASAIGYVGELCRYLM---DAPVTELDRNH-RVTKMIGNGMRPNIWDKFKQRFGVKE--VLELYASSEGNVGFSNIFNFDNTVGF--SPTPYAIIQFDKEKNEP-------IRDKNGWCQKVKAGEVGLLIGKITSRSPFDGYTDPEKNKSVIWKDVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWKGENVSTTEVENMVCEYDKIAEAVVYGVEIPNT-NGRAGMAAITLADGEELNDADLTEMVTVFKKCLPTYAVPVFLRVQKKVETTGTFKYQKNKLKEEAFNPSKTSERLLALLPGASSYCDITTEIFDNIQAYKYRF 2P2F Chain:A ((81-624)) ---------------------------------------------------------------------------CLDRHLQENGDRTAIIWEGDDTSQSKHISYRELHRDVCRFANTLLDLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSSSRLV----ITADEGVRAGRSIPLKKNVDDALKNPNVTSVE-HVIVLKRTGSDIDWQEGRDLWWRDLIEKASPEHQP---EAMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMCQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGKEKCPVVDTWWQTE-------------TGGFMITPLPGAIELKAGSATRPFFGVQPALVDNEGHPQE-GATEGNLVITDSWPGQARTLFGDHERFEQTYFS-TFK--NMYF-SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVG--IPHAIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIA--------------------------------------

General information: TITO was launched using: RESULT: Template: 2P2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2963 29292 9.89 58.82 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 9.89 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_2P2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2F-query.scw PDB file : Tito_Scwrl_2P2F.pdb: