Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFL-------------CSYFTNLPS--------------YSRFI-----ELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGK--------TSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRGY-VGKNWKSRLAEVGVQLITRV------------------KRNMKPQVLAPF-------DHAVLKKRGIIEAPFKLMKCQ---FDLEHGRHRSKMGLLTTIFAALTL--YALVLVNGYKSGIQQILKPIDLNSA 5JP6 Chain:A ((23-379)) MSQIGSSVRQAVSDNQSAQ--TLVEWENSEA--NPEALFANWRHEFMVDSSKRESMKTELCKELQALPAQDLTLFENEIRDENNRALVSGCKEELLAQVDEHFDEQRESMSVPGHALKAVQSRNSFRFPDNTQKRDMSNGYMAVRGDVARKEVVLTFDDGPHGLYTDAILRALKEVNAKAMFFATGKSVRTNPEALKRVAADGHVIGSHSITLTFDEAAAEVRGGHQAVFDVLGWVDPVFRFYGETSKDLKAFLKTKSTGEFAWNIESDDWRTQSNEQLLARVLANVESQGRGIVLFHDIQRRTAEIMPQFLRELYNRGYSVVLLTAADPSAKYNSKLVKRK--

General information: TITO was launched using: RESULT: Template: 5JP6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1124 9404 8.37 35.22 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 8.37 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_5JP6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JP6-query.scw PDB file : Tito_Scwrl_5JP6.pdb: