Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQLTNLSSAEISAQHEQDAKDLTRILPASKKVYIEGSRPDIQVPMREISLTDTPTGLGGEHNPPIMVYDTSGVYTDPNVQIDLNKGLPSVRQKWIEERNDTDVLSGLTSKFGQERLKDIRTADIRFAHIQNPRRAKAGKNVTQMHYAKQGIITPEMEYIAIRENQRQHEAVDMRQHPGQNFGAKNLKEITPEFVRQEVAEGRAIIPANINHPELEPMIIGRNFLVKINANIGNSALGSSIDEEVAKMTWATRWGADTIMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGVAENLTWEIFKDTLIEQAEQGVDYFTIHAG--VLLRYVPLTANR-------------LTGIVSRGGSIMAQWCLAHHEENFLYTHFDEICEIMKAYDVSFSLGDGLRPGCIQDANDEAQFSELKTLGELTHRAWERDVQVMIEGPGHVPMHMIKENMDLQLEVCKEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKKDVKDGIITYKIAAHAADLAKGHPGAQARDNALSKARFEFRWDDQFN---LSLDPDTARSMHDETLPKEAHKSAHFCSMCGPKFCSMKITQNVRDYANNLTNSDSEVEEGLKAMKEVYQEQGQKLYHKV------- 4IMP Chain:A ((31-550)) ---SDPAAGWFWQAVARQDLKSVSDALDLDADAPLSATLPALSVWHRQERERVLADGW---------RYRVDWVRVAP-------QPVRRTRETWLLVVPP----GGIEEALVERLTDALNTRGISTLRLDVPPAATSGELATELRAAADG--DPVKAILSL-------TALDERPHPE--------CKDVPSGIALLLNLVKALGEADLRIP-LWTITRGAVKAGPADRLLRPMQAQAWGLGRVAALEHPERWGG--LIDLP-----------------------------DSLDGDVLTRLGEALTNGLAE------DQLAIRQSGVLARRLVPAPANQPAGRKWRPRGSALITGGLGAVGAQVARWLAEIGAERIVLTSRR-----GNQAAGAAELEAELRA---LGAQVSIVACDVTDRAEMSALLAEFDVTAVFHAAGVGRLLPLAE---------------------TD----QNGLAEICAAKVRGAQVLDELC--------------DSTDLDAFVLFSSGAGVWGGGGQGAYGAANAFLDTLAEQRRARGLPATSISWGSWAGGGMADG--AAGEHLRRRGIRPMPAASAILALQEVLDQDETCVSIADVDWDRFVPTFAA---TRATRLFDEVPAARKAM

General information: TITO was launched using: RESULT: Template: 4IMP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2875 -29994 -10.43 -60.59 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -10.43 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_4IMP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IMP-query.scw PDB file : Tito_Scwrl_4IMP.pdb: