Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCCFVVYGGVLNMSDKKTSLVICFTPLQMLIAEKIVAQQPSVDLIVIALNNNDKYKYYYNIFQHPNIAKQYYLFDNNKSRFFKFINIIKFNENFKLEKFYENIYISSIDNKYTHNILSKVNFNNLYTYDDGTANIVKNSIYFKQTFESKLKDIVLKLIGIRFNLNKIKKLSRKHYTIYKGIQNIVEQTEYVSIIEKKEEDRNIFIDQEIKIFLGQPLKDID-K-------NFDTLALKKLLVM--------EEVNYHFKHPRET---DE----A-FFEEIKTSYIFEDFFAKELSKYRKVIIYTLSSTAALNIISLDNVE-IRLIKNNTVEI---KY-PDLVQLFIKSGATVIDMDSIK 2C83 Chain:A ((258-399)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QQAKFIFTGTTTWEGNTEVREYYAQQQLNLLNHFTQAEGDLFIGDHYKIYFKGHPRGGEINDIILNNAKNITNIPANISFEVLMM---TGLLPDKVGGVASSLYFSLPK-EKISHIIFTSNKQVKSKEDALNNPYVKVMRRLGIID-------

General information: TITO was launched using: RESULT: Template: 2C83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 464 15671 33.77 138.68 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 33.77 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_2C83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C83-query.scw PDB file : Tito_Scwrl_2C83.pdb: